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Figure 2
Solution X-ray scattering from S. rubiginosus glucose isomerase recorded using the MarCCD165 detector in 10 mM HEPES, pH 7.0, 150 mM NaCl and 5 mM 2-mercaptoethanol at 293 K. The 2.5 m distance and 4 mm beam stop were used. The solid curve was recorded at 1.7 g l−1 and the dotted curve at 3.6 g l−1. The dashed curve is the background scattering recorded for a matching buffer solution. Circles and squares represent the net protein scattering for 1.7 and 3.6 g l−1, respectively, after background subtraction. The inset shows the corresponding Guinier plots. The minimum Q value in the Guinier plots is 0.00552 Å−1. The radius of gyration values are 34.0 ± 0.2 Å (1.7 g l−1) and 34.1 ± 0.2 Å (3.6 g l−1).

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