SPINE bioinformatics and data-management aspects of high-throughput structural biology

Albeck, S.; Alzari, P.; Andreini, C.; Banci, L.; Berry, I.M.; Bertini, I.; Cambillau, C.; Canard, B.; Carter, L.; Cohen, S.X.; Diprose, J.M.; Dym, O.; Esnouf, R.M.; Felder, C.; Ferron, F.; Guillemot, F.; Hamer, R.; Ben Jelloul, M.; Laskowski, R.A.; Laurent, T.; Longhi, S.; Lopez, R.; Luchinat, C.; Malet, H.; Mochel, T.; Morris, R.J.; Moulinier, L.; Oinn, T.; Pajon, A.; Peleg, Y.; Perrakis, A.; Poch, O.; Prilusky, J.; Rachedi, A.; Ripp, R.; Rosato, A.; Silman, I.; Stuart, D.I.; Sussman, J.L.; Thierry, J.-C.; Thompson, J.D.; Thornton, J.M.; Unger, T.; Vaughan, B.; Vranken, W.; Watson, J.D.; Whamond, G.; Henrick, K.

doi:10.1107/S090744490602991X

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 3
An indication of the scale of results generated by high-thoughput studies. The example considers the data generated from a single plate containing DNA for studying 96 constructs through PCR, the Gateway cloning protocol (Invitrogen) and small-scale expression screens using two cell lines with each of two experimental protocols. The top half of the figure shows the usage (and therefore requirement to record in a LIMS) of 96-well and 24-well plates, the bottom half shows the number of 96-lane agarose gels, 24-well colony-plate images and 26-lane SDS–PAGE gels that also need recording. In total, a set of 96 constructs uses 34 96-well plates and 36 24-well plates and generates 480 images of colony wells, 1536 lanes on agarose gels and 416 lanes on SDS–PAGE gels.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 62| Part 10| September 2006| Pages 1184-1195

doi:10.1107/S090744490602991X

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